Confocal Microscopy System Performance: Laser Power Measurements
نویسندگان
چکیده
منابع مشابه
Laser Scanning Confocal Microscopy
Laser scanning confocal microscopy has become an invaluable tool for a wide range of investigations in the biological and medical sciences for imaging thin optical sections in living and fixed specimens ranging in thickness up to 100 micrometers. Modern instruments are equipped with 3-5 laser systems controlled by high-speed acousto-optic tunable filters (AOTFs), which allow very precise regula...
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Many technological advancements of the past decade have contributed to improvements in the photon efficiency of the confocal laser scanning microscope (CLSM). The resolution of images from the new generation of CLSMs is approaching that achieved by the microscope itself because of continued development in digital imaging methods, laser technology and the availability of brighter and more photos...
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A new nondestructive technique, confocal scanning laser microscopy (CSLM), is described which is used to characterize the topography and morphology of polymer coatings. The topography of the coating can be determined even when the coating is completely opaque. When the coating is not completely opaque, CSLM has the distinct advantage of also being able to distinguish between the coating surface...
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Optical technologies are being used increasingly to perform real-time assessment of tissue abnormalities. Many groups have demonstrated that quantitative optical spectroscopic approaches, including fluorescence spectroscopy1-4 and reflectance spectroscopy,5, 6 have the potential to deliver a highly sensitive, specific, and costeffective diagnosis in real time and without the removal of tissue. ...
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ژورنال
عنوان ژورنال: Microscopy Today
سال: 2002
ISSN: 1551-9295,2150-3583
DOI: 10.1017/s1551929500058466